LOCUS       EY193364                 273 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa93d04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193364
VERSION     EY193364.1  GI:159496110
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 845 was extended to value 846 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 465
            PolyA tail found inside read...SR being truncated to 424
            Potential full insert read...vec-to-vec length: 345
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc acttttgatc atgatctctc cgccaaagat gaacttgcta aacgacaata
       61 atgagttccg acacctgggc catttcctaa caaacaacga cgcctccgac ccattccaca
      121 agaaagtgct gcgccaccac gaacaagcct gtcagttgct cgcctccaaa tgggatgcgc
      181 tggagtggac gaaattttct cctgtgttgt gcgaagagga aaaagatggg aagcggcaaa
      241 gaggggacaa gcagaggact cgttctccaa cca
//