LOCUS EY193356 190 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa93c08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193356 VERSION EY193356.1 GI:159496102 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 190) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 845 was extended to value 845 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 385 PolyA tail found inside read...SR being truncated to 342 Potential full insert read...vec-to-vec length: 262 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..190 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcgc tcattctccg agcaaggcat cacatcgggc gaaacagtgc tcatcaccat 61 gtaagcagaa tgcggagcag aaggagcgga accaggaacc agcccaaacc aattgatacc 121 gatcggaaat ataattaatt atacaatatg catgcattcg acatttcata ccaatcccca 181 caaagcaata //