LOCUS       EY193352                 486 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa93c04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193352
VERSION     EY193352.1  GI:159496098
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 675
            PolyA tail found inside read...SR being truncated to 637
            Potential full insert read...vec-to-vec length: 558
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtgc ctcgagccga tttgctcaca ccttcatcgc ctcaagaagc tgaccctttc
       61 tcggtgctat gggattgagc cggtgggctt cttgtcgctg ggccacttga aggagctaaa
      121 cgtctacggc tgcatcactg agaacggggt gaacgtgctg cgcaaccgac tggcgcccac
      181 caaaatcaac gcctccccct tttcgacgat tgccaagccg acagtgggcg aatccatatc
      241 cagcatttgg caggaacgca ccagagacct ttactgactg accaaaatgg ggagatgaat
      301 gaggatgaac gaggtggatg gaatacgatg cttgcactcg ctgccttgat cctaacttta
      361 ctccttgcta tatttgcctg atctgaactc ttttcctctc gaccatctct gcaatttgaa
      421 ctttaaagtg accaataaaa ttattgactg cttgccctgg aaaaaaataa actcgaccat
      481 ttaaga
//