LOCUS       EY193349                 272 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa93c01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193349
VERSION     EY193349.1  GI:159496095
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 844 was extended to value 844 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 465
            PolyA tail found inside read...SR being truncated to 423
            Potential full insert read...vec-to-vec length: 344
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggag gaagcggacc gactgaaaaa tgaaaccgga acagggacga ctgtagatga
       61 agaaccaaca acgcaatggg gggcagagga agacagccct gtgcctctta ttagcgaggc
      121 cgaagagaag caaattctgg aaaaagggag agcgcggcgc atcaagtttg acgattgatg
      181 gggagtaagg aagatccaac ttgactgacc atctcatttt catttctcat ttgttgttgt
      241 gtctatcgcg aattataaaa attaaaaatg ga
//