LOCUS EY193346 575 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa93b08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_506709.1| Putative cytoplasmic protein of eukaryotic origin (32.3 kD) [Caenorhabditis elegans] pir|T19448 hypothetical protein C25D7.8 - Caenorhabditis elegans emb|CAB02772.1| Hypothetical protein C25D7.8 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY193346 VERSION EY193346.1 GI:159496092 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 575) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 863 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..575 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtga cgaacaaact agtccccagt acttccagaa agcgacggaa cttgccacca 61 tttattcgca tattcgtcgg attcgcggcg atgggaattg tttttaccgc gccgttctaa 121 cagcccagct cgagcgatgc tttgacgacc taaacgaatt gaaccgattc aaggaattga 181 tcaaaggctg gcgaaaacgg ctaatggatc tgggctctta ccccgaattg accaccggcg 241 acttttgcga ttcgatggag acactcatgg actcgatgct ggacgggaca atgcagcccc 301 agatcctgat ggacgacctg gaccaggatg gggtggccaa ctattacgtc gcattcaccc 361 ggctcatatg ttctggctat ttgcgcgaaa acgaggccct ctattcgggg ttcatagagg 421 gcggcaagac actggaccaa ttctgtcgag acgagatcga gccaatgtgg aaggatgcag 481 acgccgtttg cataattgca ctggtcaacg caattggcgt ttcgatccgc atcgagtaca 541 tggaccgaag tcaggcgccg agtggcggtt ggcac //