LOCUS       EY193342                 323 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa93b03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193342
VERSION     EY193342.1  GI:159496088
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 845 was extended to value 845 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 515
            PolyA tail found inside read...SR being truncated to 473
            Potential full insert read...vec-to-vec length: 395
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggcctggggg aacagaggac tactgctcag caaaagggtt ccaccagtga acagtccccg
       61 actgtcgacg tgccaaccga aaacattgct tcgcgtgcgg atgaggcctt gctgttgcag
      121 caaaaacgcc gccagagcga aaagtcgcgt tgggagaatg cgcgcaaacg cagggccgaa
      181 ctgatgggtg ccaactactt tgacgaggag gcgcagaaat gaagaaagaa tacataaatg
      241 actgaacatg gcgggttatg ttgtaatttt cagcacatta ttgactcggc tgtttgaaat
      301 atggcaaaag acaatttgtt cca
//