LOCUS       EY193340                 616 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa93a11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to sp|P30163|ACT2_ONCVO ACTIN 2 gb|AAA29410.1| actin 2,
            mRNA sequence.
ACCESSION   EY193340
VERSION     EY193340.1  GI:159496086
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 817 was extended to value 817 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 820
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg acagaaggac tcgtacgtcg gagatgaagc ccagtccaag cgtggtattc
       61 tcaccctgaa gtacccgatt gagcacggca tcgtcaccaa ctgggacgac atggagaaga
      121 tctggcacca cactttctac aacgagctgc gtgttgcccc tgaggagcac ccagtgctgc
      181 tgactgaggc accgctgaac ccgaaggcca accgtgagaa gatgacccag atcatgttcg
      241 agaccttcaa cacgccggca atgtacgtgg ccatccaagc cgtgctctcg ctctatgcgt
      301 ccggacgtac caccggcatt gtgctcgact ctggcgatgg tgtgacccac accgtgccca
      361 tctacgaggg ttacgcgctg ccgcacgcca tcctccgtct ggacttggcc ggccgtgatc
      421 tgaccgacta cttgatgaag atcctcactg agcgcggtta ctcgttcacc accaccgccg
      481 agcgtgagat cgtccgcgac ataaaggaga agctctgcta cgtggcactt gactttgagc
      541 aggagatggc caccgccgcc tcctcttcgt cgctggagaa gagctacgag ttgcccgacg
      601 gacaggtgat caccgt
//