LOCUS EY193332 534 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa91h10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|XP_313694.1| ENSANGP00000013133 [Anopheles gambiae] gb|EAA09249.1| ENSANGP00000013133 [Anopheles gambiae str. PEST], mRNA sequence. ACCESSION EY193332 VERSION EY193332.1 GI:159496078 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 534) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 783 was extended to value 784 (,) putative full insert read: vector-to-vector length is 600 (,) This trace has been recalled with phred original value before phred recall for SL was 147 original value before phred recall for SR was 747 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..534 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtgc gcatgagcga ggcgctcgcc aaaatggagc tgcagccgtt tgcggaggag 61 cgccacgtgg acgaggcgct ccgtctgttc aaggtgtcca ccattgcggc ggcgcagagc 121 ggcaatctgg caggcgtgga gggcttcagc tcggccgagg accagcagca gttcagtcgc 181 gtggagcgcc agctgaagaa gcgcttccaa attggcactt acgcctccga gcagttgatc 241 atttcggagt ttgagcgcca gagctatccg gaggcactcg tgcgaaaggc cattgagtat 301 tgtgtgcgtc gcggcgagct ccagtaccgg atgcagcgca aaatgttgta ccgcgtgaaa 361 tgatgcgctg aaggcgcaca gaaggatggt ggatgatgga tggatggata tgcctgcacc 421 agttttaaca attatacgca tctcgacaac cattttcatt cttggaattt ttggaagcca 481 acaacacttt cgactgaaaa catgtccaac acaaattttg tgaaaactac aagt //