LOCUS       EY193325                 449 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa91h02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193325
VERSION     EY193325.1  GI:159496071
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 641
            PolyA tail found inside read...SR being truncated to 601
            Potential full insert read...vec-to-vec length: 521
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggag ggaagaagcc ctgaagttac aacgctctgc taagaccaag gaggagagca
       61 gcgcagcatt tgcccgattc gaattggcca gagaagcgct tgaacagctg gacgcgcatt
      121 ccggcgcagg cgtgacctac gagaacgttc ctactgccag gaagactgcc tttgatcgtc
      181 taggagcact cttcaagacc atccgtggag cgaagaaggt atataaggct taaaccatat
      241 cattttcata tttcccttga gacggatgct cagtcatctg ctaaggcaaa atgcgcaaat
      301 gacgttgaca aggacgagca atcttctgaa gggacagtat atgcaagtaa gcatggactg
      361 ctaataaaac actaattaat gattattgat cttccacgat cccgattaat ttgtttgtac
      421 tctgggcagt tgataaagac acgcgatta
//