LOCUS       EY193321                 390 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa91g08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193321
VERSION     EY193321.1  GI:159497452
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 585
            PolyA tail found inside read...SR being truncated to 544
            Potential full insert read...vec-to-vec length: 462
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc accagatcaa cgtgcagcag ctgtacgcgg cgctggacga gctcggaatg
       61 gagctgttcg tgaaacaaga gaaccaccgt ctgcagggcc acaagataac cggcgagctg
      121 gccccaccgc tggcaagatc tggcgcacac gttcagcgtc ttcaatccgg agaacattgg
      181 cgggcggctc aagtacgtgc tgaagagcgc cctatacgag caggcggaag tgaaggacca
      241 catcagggcc gcactccgat tttgcgatag gacgagaaga gaacaaaatt gtgtttttca
      301 agatgacaaa attgtgatga attcccacgt ttgacaataa taaacactga tattgtttca
      361 attttttacc cggataaaca ggttgtacga
//