LOCUS EY193318 263 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa91g05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193318 VERSION EY193318.1 GI:159497449 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 263) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 457 PolyA tail found inside read...SR being truncated to 414 Potential full insert read...vec-to-vec length: 335 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..263 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa taatcctatg cttttcaagt ccacagcaaa atattgggct cagcattatg 61 cgaacgctcc tggagaaatg aatacggata tgctcaaaag tgtgaccaag ttgagagaaa 121 tgggcgcagc tgtggatgtg tgtatttctg cgttgagctg tcatgactgg aatctgcaga 181 aggcgacgca atcgatattt aattgaaagc attttatcac caaacttctc agtgtttgaa 241 ctttgaagtc agagacaatg cca //