LOCUS EY193317 201 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa91g04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193317 VERSION EY193317.1 GI:159497448 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 201) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 808 was extended to value 808 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 393 PolyA tail found inside read...SR being truncated to 353 Potential full insert read...vec-to-vec length: 273 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..201 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggat tccaacggca ccgtctagac caaatcggga agtggggact gcgagccggc 61 actgattgtc ccaaacctgc gattatcact gtccaaccat gtcgctctgt tttgcccctt 121 ttgttgagcc ccgagtttct tgttggctgt tgatgtgtgt ctgttcattt cggcggttgg 181 ttattaaaca ttgagtgggg a //