LOCUS       EY193313                 232 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa91f12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193313
VERSION     EY193313.1  GI:159497444
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 790 was extended to value 790 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 425
            PolyA tail found inside read...SR being truncated to 383
            Potential full insert read...vec-to-vec length: 304
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggag cagaaaaaca agtgcgcgca aatgcaaagc gaactgaaca cttccgaggc
       61 cgtccagaaa ggtcagcaga ttgagtgaaa cacagggctg cacattgccc cacttttcgg
      121 accatttcgg tgtgtttcca attttcacct ttccggtctt ttgtccattt tgtcactcgt
      181 gaactgtatg cgtttgtcac atgtatcgtc ggtaaaaaat ttgaaagtgg ca
//