LOCUS       EY193309                 321 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa91f08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193309
VERSION     EY193309.1  GI:159497440
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 514
            PolyA tail found inside read...SR being truncated to 471
            Potential full insert read...vec-to-vec length: 393
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggt tatcgaaatg aagaatattc tctttctcct ctgcctcgcc attttggcgg
       61 ttttcatcgc tgtcggccaa ggccaaggcg taaattcacc gtttgacggc tcgcacccga
      121 ctggcggcaa actaagcgac tacttgctgt gcttgaagaa gtaaaacact agcagctaca
      181 ttacacattc aattggaaaa gaagctggaa atgtgcgaat cattcattaa ttaatcattt
      241 actgtttact cattagttgt tcactcgttt atttcgatat gatgtctgat gtgtataaaa
      301 ggataaaatg gattgttact a
//