LOCUS       EY193306                 444 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa91f05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193306
VERSION     EY193306.1  GI:159497409
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 824 was extended to value 824 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 640
            PolyA tail found inside read...SR being truncated to 597
            Potential full insert read...vec-to-vec length: 516
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcac tccattcagc gacaaccggt ctgtccaaca gcggccgaga tctggtccaa
       61 accgaagcgg agaaacacgc caccattctg atgcaatatc tgaggtgttg ccatggcgat
      121 ttgggggcgg ccaagcgtta cgcggagctg ctgctgctgg ccgaagaggt gttccacgac
      181 gctcaccgcc acctggactt tgccacgtat ttcgcggcca ccaacgaagg cgctaggagg
      241 gccctgaatt ttccgccagc actgggccca atgctctggc acgactgcaa ccccagtccc
      301 aaaaacggga ggtgaagtga agacatccga gagaattgag atcagagatc actcactgtc
      361 actggtcatc catcggcagg actaattctt cgaatacttt catcctgtcc tctattttga
      421 tttgaaataa atccacgatt taca
//