LOCUS EY193278 580 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa91d01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_499054.1| Transthyretin-like family member precursor family member [Caenorhabditis elegans] sp|Q03575|YLF5_CAEEL Hypothetical protein C40H1.5 in chromosome III precursor pir|S28300 hypothetical protein C40H1.5 - Caenorhabditis elegans emb|, mRNA sequence. ACCESSION EY193278 VERSION EY193278.1 GI:159497320 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 580) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 810 was extended to value 811 (,) putative full insert read: vector-to-vector length is 603 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 753 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..580 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac tccgaaattc acaggcgtaa acagagccaa tcacaaaatg cacccggtgt 61 tggtcgtgtg tctggggctc tcgctggcgg ccctcggtga ggcgggcgtg ggcttgctcg 121 gctccgagca gtcggccgca gtccgggggg tgctgatgtg caacgggcgg cccagtgtgg 181 gcaccaaggt gaagctgtac gacgaggacg acaccgacat ggacgacctg atggcggagg 241 gggtcacgga ccgccagggc cggttccagc tgagcgggca cgtcaaggag gtcaccacca 301 tcgaccccaa actgaacgtg taccacgatt gcaacgacgt ccatgtgccg tgcaaactga 361 agttctcgat caagctgccg gacgggtacg tgagcaaggg cactgtggcg cgcaaagtgt 421 tcgacgctgg cacgctgaac ctggagggcg ccttctccgg tcagagtcgc gattgcgtca 481 actgaacgga agggaggaac aacgaattca aactagaaac cctcgcaata tcctaaaacg 541 caataaattg gtgagcgcag agtgaaaaaa aaaaaaaaaa //