LOCUS       EY193277                 433 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa91c12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_497810.1| DNA helicase [Caenorhabditis elegans]
            pir|T20430 hypothetical protein E03A3.2 - Caenorhabditis elegans
            emb|CAA86232.1| Hypothetical protein E03A3.2 [Caenorhabditis
            elegans], mRNA sequence.
ACCESSION   EY193277
VERSION     EY193277.1  GI:159497319
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 627
            PolyA tail found inside read...SR being truncated to 584
            Potential full insert read...vec-to-vec length: 505
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtac caggagagcg ggagggctgg cagagacggc aagcgcagct acgctcgcat
       61 ctactactcg cgcgacaatc gcggaatgat ggactacagg ctgcagtgct cgctctattc
      121 cgtcgagcag aaaacgaata caccgaagga actgatagag caacgcaaag aggagataca
      181 aaggggtttt aggcggatga tcgaatattt ggagagaagc accgagtgca gacacaaatt
      241 catcggccac tattttgacg acgacgaagt gaatccgtgc aaagaaaact gcgactgttg
      301 cgaagtgccg gaaaaagtaa gcgaaggatt gaagagatta gctcattcac aataacactt
      361 gagacaacat cactatttga ttattgataa atctattctt gtttaaactt ttaaataaag
      421 gcttaatttc cca
//