LOCUS EY193275 207 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa91c10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to sp|P12890|AMD2_XENLA Peptidyl-glycine alpha-amidating monooxygenase II precursor (Peptide C-terminal alpha-amidating enzyme II) (AE-II) pir|URXLA2 peptidylglycine monooxygenase (EC 1.14.17.3) II precursor - African clawed frog gb|AAA49667.1| alpha, mRNA sequence. ACCESSION EY193275 VERSION EY193275.1 GI:159497285 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 207) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 820 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..207 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtac ctgccccacg gactgacctt cgatgcggaa ggcaacctct ggctgacgga 61 tgtgggcttc caccatgtgc acaaactgga caagcagttc ttgccgctga ttactctcgg 121 cgagaattcg gggccgggtt ctgacaacaa acacttctgc aacccaaccg atgtggctgt 181 ggccgccacc ggcgacttct tcatcgc //