LOCUS       EY193255                 279 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa91a12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193255
VERSION     EY193255.1  GI:159497207
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 386 (,)
            original QR value of 734 was extended to value 734 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 535
            PolyA tail found inside read...SR being truncated to 431
            Potential full insert read...vec-to-vec length: 351
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa gcgcgaagac aattcggaca agccattcgg cgtttcggtc gaagtgcacg
       61 agaaactgat ggaatgcttc ggcaaattcg cctacgatga ttgcgcaaat cgcaatgaat
      121 gctgcgagaa gttccacaca actggatgca cttgctgaaa caatgatgat gagaagacat
      181 aggagagagt ggaacagaga aagtaattga aatttgaaag tttgtcgaaa tctggataca
      241 aatcgaattt tgttttttga acattaaatt agtcgcgga
//