LOCUS EY193238 471 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa90h05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_573005.1| CG9091-PA [Drosophila melanogaster] sp|Q9VXX8|R37A_DROME Probable 60S ribosomal protein L37-A gb|AAF48428.1| CG9091-PA [Drosophila melanogaster], mRNA sequence. ACCESSION EY193238 VERSION EY193238.1 GI:159497162 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 471) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 826 was extended to value 826 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 697 PolyA tail found inside read...SR being truncated to 622 Potential full insert read...vec-to-vec length: 543 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..471 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggga tgcgctgatt ggccagctga agttcactcc gggcatgggc cgtcgtggag 61 cgggctggac cgaatcggat gccgccggac aacgcaactg gaatgtggtg aaattccgcg 121 cccactggac gccgaagttt cgactgttgc ttgatgcatt gtgcaaactc cgctcacagc 181 actcacgtca acttgagcac ccaacaggag gcgttggccc tgcccatgtg tagctcagaa 241 cacactacca cccaaactgg gcgtgccgtt gccgccgccg cagcaggacc gtcacggcac 301 caacagctga atgtgaggca acgccgcttc cgcaacggat tccgcgaggg cactgtggcc 361 aaatcgcaga aagggcaaga gcgcctagac gccggagatt tgaaccagaa cattgttttg 421 atggattgtt gcgaaattgt tgttaacgag aaatgaaaaa tacttccatt a //