LOCUS       EY193069                 571 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa88g07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_498596.1| Prolyl tRNA synthetase PRS-1 (65.8 kD)
            (prs-1) [Caenorhabditis elegans] pir|T16915 hypothetical protein
            T20H4.3 - Caenorhabditis elegans gb|AAA50660.1| Prolyl trna
            synthetase protein 1 [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY193069
VERSION     EY193069.1  GI:159496432
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 812 was extended to value 812 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 814
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtgt ccgagtggag accgatctgc gtgacaatgt atcgcccggt tggaagttca
       61 accactggga gttgaagggg gtgcccattc gcctggaggt tggcccaaaa gacttggcca
      121 aggagcagac tctggccgtt ctgcgctatt ctggcgagaa gcgtcccctg ggtctggcca
      181 atctgaactc ggaggtcaaa gagttgctgg agttcatcca ccacgaaatg tacaagagaa
      241 ttgagcaacg gcgtgatgcg aacacgcaca tctgcttgga ctgggcacag ttcactccac
      301 agctggacaa caaggcaatc ctgctttcgc cattttgcgg cagaggagac tgcgaaacgc
      361 tgatcaagga gcagagtacc agggaagaac cggccccgga tcagatcggc acatcccaaa
      421 tgggcgccaa gacgctgtgc attccgctgg agcagcccaa gaacgtgcaa ttgcccgaaa
      481 agtgcatact tcccacctgc acccacaagg cacagttttt cgcgttgttc ggacgaagct
      541 actgaattga cgctttcgta ttgctgagtt g
//