LOCUS EY193060 372 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa88f07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193060 VERSION EY193060.1 GI:159496395 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 372) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 792 was extended to value 792 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 565 PolyA tail found inside read...SR being truncated to 523 Potential full insert read...vec-to-vec length: 444 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..372 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtc tcaaagaact acttcatgcg gcagctcaaa gagcccaagg aggaatacgt 61 gatcagcgag gtgggcatct acggctcatt gattgccaac cagaagagcg gcgagatcct 121 gcacaacgat gtcgacgggc atctgtgcag gagcaagccc gccgagttca acgagggcgg 181 agtgagtggc ggcggcggtg tgatcgactc cgtgttgttg ttccctgcca gcgatttcca 241 atgaatttga tcaattttta aagactaccc gaaccaatat tgcgctcaac aatttgggat 301 attcgttcat caatcgtcat attgctcgct ctcattttat tctagtagtt agcataaagt 361 gttaaaaaat ca //