LOCUS       EY193057                 272 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa88f04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193057
VERSION     EY193057.1  GI:159496392
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 824 was extended to value 824 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 465
            PolyA tail found inside read...SR being truncated to 423
            Potential full insert read...vec-to-vec length: 344
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcac gcaggagact acagtgagca tccagatcgc gactattagc atcttctgtg
       61 gctgcaattg aggcgcaaga cagaagagag tggcgtccaa cggattcctt tctcgaggaa
      121 gtcaccacgt gttgagccgc accttttctc tccagcactt tcctgtgcat agtcctaaaa
      181 gaggcaagac atcgcccttt gacccaaaac gacagaacac cgccgctttt tgtagttttc
      241 ttacactttc cttgtgaata aatggtgcgt ga
//