LOCUS EY193053 341 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa88e12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193053 VERSION EY193053.1 GI:159496388 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 341) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 787 was extended to value 787 (,) This trace has been recalled with phred original value before phred recall for SL was 152 original value before phred recall for SR was 538 PolyA tail found inside read...SR being truncated to 492 Potential full insert read...vec-to-vec length: 413 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..341 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggag gagtgtcggc accacaacgg tgtggagaca caacttgcag ctgagggatc 61 actgctcagc ctcatcccac gcagtgcgac cccactatcc gacttgcacc ccagcgatgg 121 ctcatccgcc cagcagcaga aatcgtcagc aacaaccgtc ctccccaacg gccgataccg 181 ggtgcgaaaa gacggagagg caatgccaaa aagaattgac ttacgtgtga ttcgaataaa 241 atcattcgcc aaaaacggtg ctcaatcgcg gatgcctgtc tgtacatatt gagactttcg 301 atgtgaatat tatgtacaaa cgttagaata aagaatctgc a //