LOCUS EY193050 369 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa88e09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193050 VERSION EY193050.1 GI:159496356 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 369) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 823 was extended to value 824 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 562 PolyA tail found inside read...SR being truncated to 520 Potential full insert read...vec-to-vec length: 441 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..369 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggat gacccacatc gtgaatagta ccgatccaaa ctatgtgatg aaccttccgg 61 aaccgactga agacacgctt gcggctatca ttgaattgga ggcgaatact cgtgtggtcg 121 cgggcaatat cctgggcgaa gtcaccggtg acggaggcca tttccgagac aatgtgccac 181 tgttgcgttt gacggccaat gttctggaca cattcgacgg gcatttggca attgcgccac 241 tgaggtgaag aacgaaggca gtgaggatat ttttcaatgt taacgtcttg aacatgttta 301 tctttgttga aaataacata agtcttattt tatttttatt aagtaggcac aaaagaaatt 361 tgcaccaga //