LOCUS EY193049 133 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa88e08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_491233.1| RAB family RAB-2 (23.6 kD) (rab-2) [Caenorhabditis elegans] pir|T25796 hypothetical protein F53F10.4 - Caenorhabditis elegans gb|AAB52431.1| Rab family protein 2 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY193049 VERSION EY193049.1 GI:159496355 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 133) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 833 was extended to value 833 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 326 PolyA tail found inside read...SR being truncated to 285 Potential full insert read...vec-to-vec length: 205 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..133 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcca gacgtgatac cttcaaccat ctgacctcgt ggctggagga cgctcgtcaa 61 cattccaact cgaacatggt catcatgcta atcggaaaca agagtgattt ggaagcaaga 121 cgtgaagtta ata //