LOCUS       EY193035                 464 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa88d03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to gb|AAC08347.1| 60S ribosomal protein L13a [Ostertagia
            ostertagi], mRNA sequence.
ACCESSION   EY193035
VERSION     EY193035.1  GI:159496312
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 657
            PolyA tail found inside read...SR being truncated to 616
            Potential full insert read...vec-to-vec length: 536
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcac cgtcgcgtat cttctggcgc acggttcgtg gcatggtccc tcacaaaact
       61 gctcgtggga tggaagcgct gaagaaactg aaatgttttg acggctgtcc ccagccctac
      121 gacacaaaga agaaattcgt tctacccaac tctcttcgcc acatcgcact caagccgcgt
      181 cgcaaatgct gcactgttgg acgtttggcg cacgaggtcg gttggcagta ccaaggcgtc
      241 gtggagaaac tggaggccaa gcgcaaagtg cgtggtgccg ccttccatga gaagaagaag
      301 gccgagcaga agctgaagaa gcaggcattg gagaaatcaa aatccaagac tgccccgttc
      361 gacaagattt tggcgcagta cggatacgcc taaattggct tcttatttgt tgatgattgt
      421 tctgttgaaa ttttgaatcg agtaaattcg tggagattca aaca
//