LOCUS       EY193032                 519 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa88c12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_187574.1| 60S ribosomal protein L4/L1 (RPL4A)
            [Arabidopsis thaliana] sp|Q9SF40|RL4B_ARATH 60S ribosomal protein
            L4-2 (L1) gb|AAF23293.1|AC016661_18 putative 60S ribosomal protein
            L1 [Arabidopsis thaliana] gb|AAL09727.1| AT3g09630/F11F8_22 [, mRNA
            sequence.
ACCESSION   EY193032
VERSION     EY193032.1  GI:159496280
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 561 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 709
            PolyA tail found inside read...SR being truncated to 670
            Potential full insert read...vec-to-vec length: 591
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg ccttccgcaa cattcctggg gtcacactgc tctctgtcaa ccgcatcaat
       61 ttgctgaaga ttgctcctgg tggacatttg ggacgtctcg ttgtgtggac cgagagtgct
      121 ttccgcaaat tggattctat cttcggcagc tttaccagga aatctgatgt taagaagggg
      181 ttcactctgc cccgcgcaaa aatgaccaac tccgacctcg tgcgtatcat tcgctcggac
      241 gaaattcaga aggttgtgcg cccgatcaag aagcagagga aggtcgcaaa ggttcaccgc
      301 aatccgctga agaagcactc tttgatggtc aaactgaacc cctatgcttc cgttcaacgc
      361 agagccgcaa ttatcgcgtc ggctaagggg caacagcaaa aggcctagaa gaatgaattg
      421 agtgggtacc ggacaaacat cagctggcgg aaaattcgaa tgttgaccat ttttgttggc
      481 aaaattgtta tttcatgttg caaataaact gcacggaca
//