LOCUS       EY193030                 577 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa88c10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_508841.1| Actin ACT-4 (41.8 kD) (act-4)
            [Caenorhabditis elegans] sp|P10986|ACT4_CAEEL ACTIN 4 pir|S27135
            actin 4 - Caenorhabditis elegans emb|CAA34720.1| actin
            [Caenorhabditis elegans] gb|AAB04575.1| Actin protein 4, isoform a
            [Caenorhabd, mRNA sequence.
ACCESSION   EY193030
VERSION     EY193030.1  GI:159496278
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 800 was extended to value 800 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 802
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcgt gagatcgtgc gcgacatcaa ggagaagctg tgctacgtgg cgctggactt
       61 cgagcaggag atggctaccg ctgcctcctc ctcctcgctg gagaagagct acgagctgcc
      121 cgacggtcag gtgatcaccg tcggaaacga gcgcttccgt tgcccggagg ccctcttcca
      181 gccgtccttc ttgggcgtgg agtctgccgg catccacgag acgtcgtaca actccatcat
      241 gaagtgcgac atcgacatcc gtaaggacct gtacgccaac accgtgctct ctggcggcac
      301 caccatgtac ccgggaattg ccgaccgcat gcagaaggag atcaccgctc tggccccgtc
      361 gacgatgaag atcaagatca tcgctcctcc tgagcgcaag tactccgtct ggatcggagg
      421 ctccatcctg gcctcgctgt ccaccttcca gcagatgtgg atcagcaagc aggagtacga
      481 cgagtcgggc ccgtccatcg tgcaccgcaa gtgcttctaa gcagggacag cggacagcga
      541 gacagacacg gaggaaggca gacagacaga caacaga
//