LOCUS       EY193027                 243 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa88c07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193027
VERSION     EY193027.1  GI:159496275
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 825 was extended to value 825 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 440
            PolyA tail found inside read...SR being truncated to 395
            Potential full insert read...vec-to-vec length: 315
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggtc ccgcgggcac aagaaacaga agcggaatga aaactgtcca cggaatggcg
       61 aactgtccgt cccctgtccc ctctctcctc gtgcactgat ttggccattg cctgacaacc
      121 accaaatatg tccatccccc ctacttctcc tccgctgcta tctattctcc tcctctgacc
      181 accgatcgat gctgaagact tgacaacatt ttgcaaatgg cgagcataaa ccagttggga
      241 gca
//