LOCUS       EY193018                 497 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa88b09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_492457.1| Translation elongation factor 2 EFT-2
            (94.8 kD) (eft-2) [Caenorhabditis elegans] sp|P29691|EF2_CAEEL
            Elongation factor 2 (EF-2) pir|T21362 hypothetical protein F25H5.4
            - Caenorhabditis elegans emb|CAB02985.1| C. elegans EFT-2 pro, mRNA
            sequence.
ACCESSION   EY193018
VERSION     EY193018.1  GI:159496241
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 809 was extended to value 810 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 814
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga ccggaaggaa ccggtgccaa cattctcgtt gatgttacca agggagtgca
       61 gtatctgaac gagatcaagg attccgttgt gaccggtttc caatgggcca ccaaggaagg
      121 cgtgctctgc gacgagaaca tgcgtggcat ccgtttcgac atccacgacg tcactttgca
      181 tgccgatgca atccaccgtg gcggcggaca gatcatcccg actgcacgtc gcgtgttcta
      241 cgcttcagtc ctgaccgcca agccacgcct tctggagcct gtttacctgg tggaaattca
      301 atgtccagag gcggcagtgg gaggcattta cggagtgctg aaccgtcgcc gtggcgttgt
      361 atttgaggaa agtcaaatcg cacgcacccc aatgttcatc gtgaaggcac atttgcccgt
      421 caacgagtct ttcggtttca cccgctgatc tgcgttctaa caccggcgga caggcattcc
      481 cgccatgcgt gttccac
//