LOCUS       EY193014                 451 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa88b05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_499884.1| Alanine dehydrogenase/pyridine
            nucleotide transhydrogenase and Saccharopine dehydrogenase (103.8
            kD) [Caenorhabditis elegans] pir|T15063 hypothetical protein
            R02D3.1 - Caenorhabditis elegans gb|AAB94142.1| Hypothetical
            protein R02, mRNA sequence.
ACCESSION   EY193014
VERSION     EY193014.1  GI:159496207
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 645
            PolyA tail found inside read...SR being truncated to 602
            Potential full insert read...vec-to-vec length: 523
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggt gccgcatctt gcaaaagctt tggcgtatga gcaggcggaa cgcgatctga
       61 tcattttgaa ccatgacatc gaggcacaac ttccatccgg ctcaattgaa aaacacagaa
      121 tcagcttggt ggcttacggt gaacagcaca acggcggcta ttcagcgatg gccagaacag
      181 tcgggttcac agctgcttta gtatcacaaa tgattttgga cggcgaaata cagcaaaagg
      241 gaatgctgag gccaatttcg aaggctgtgt acagacctgt actccagaga ctccaagatc
      301 ttggcatcaa ggggaccaac aaagtgatag tcagtcagtg atggttcagg ctcaaaaatg
      361 ttttccacac cccccacttc ccatttattt gttcactctt tcattgtgct gtaatttaaa
      421 ccatattttt taaattaaaa tccaattacc a
//