LOCUS EY193006 378 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa88a08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_497701.1| Mitochondrial glycoprotein (26.4 kD) [Caenorhabditis elegans] sp|Q21018|YMI3_CAEEL Hypothetical protein F59A2.3 in chromosome III, mitochondrial precursor pir|T22972 hypothetical protein F59A2.3 - Caenorhabditis elegans emb|CAA84, mRNA sequence. ACCESSION EY193006 VERSION EY193006.1 GI:159496168 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 378) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 574 PolyA tail found inside read...SR being truncated to 530 Potential full insert read...vec-to-vec length: 450 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..378 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcac aagagcagcg gagggagtgg aggatgagcc ggtcgatgac cgggtctaca 61 tgtcttctgg caagtacatc gacccgaaat tgcacgactt gctcttcgtc aaatatttgg 121 aagaacgcgg atttaccgcc gaattctgcc accaacttgt ggtcttctcg actcactatg 181 agcacagcca gtacgtgaat ctgctggccg ctatcagaaa gtttgttact tcggcatgaa 241 gtgctagaag agcgattgga gaaaaaaaaa gatcccgtgt tgttgattta tttgctcgcg 301 ttttacacac ttagaccaag ctttgaccac tccatggaaa gtctggaccg tcttcaaaat 361 aaacgttttt tgatacca //