LOCUS       EY193003                 462 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa88a05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_491440.1| Rho-associated kinase, LEThal LET-502
            (135.8 kD) (let-502) [Caenorhabditis elegans] pir|T25539
            hypothetical protein C10H11.9 - Caenorhabditis elegans
            gb|AAB42081.1| LET-502 [Caenorhabditis elegans] gb|AAB42348.1|
            Lethal protein 5, mRNA sequence.
ACCESSION   EY193003
VERSION     EY193003.1  GI:159496165
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 839 was extended to value 840 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 656
            PolyA tail found inside read...SR being truncated to 613
            Potential full insert read...vec-to-vec length: 534
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaag tgacgacgac acaacccatt ttgaagatgt ggaacaggcc gatccagtca
       61 actcggaatc cttccaaatt cccaaggcgt tcaccggcaa tcaactccca ttcatcggtt
      121 tcacgtactc caacgaattt gggccattgg aaattattcg aaagcaaatt ggaatgaacg
      181 gcgtaatcca taacgggctg gttgcgccca ctatagccgt gcagatggaa aatgcaacca
      241 aatcagccaa tgatgccgag atggagcagt tgagaggcga aaagcgctta ctaggatcac
      301 atgttgctga gttgcagcag aagatgtcgg acgcagaagt tgcagcgaaa ttggaggccg
      361 actcactcag acgagacaag gaatacctcg aattgcgtac aaacgaactc aacaatgagc
      421 tgaatcacat ttccaaacaa gaaattgaga agttgaagaa ca
//