LOCUS       EY192999                 607 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa88a01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to pir|E81737 hypothetical protein TC0128 [imported] -
            Chlamydia muridarum (strain Nigg), mRNA sequence.
ACCESSION   EY192999
VERSION     EY192999.1  GI:159497128
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 817 was extended to value 817 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 827
            COMM   possible reversed clone; protein similarity on positive
            strand
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtct gcatatgtta catatagtca ggtaagtaaa ttataaagag cacttgatgg
       61 atgccttggc gttaagaggc gatgaaggac gtggcaggct gcgataagct gcggggaact
      121 gtcaacaagt tttgatccgc agatttccga atggggaaac ccaactagct tagctagtta
      181 ttttatgctg tataagtatg aaaggtaaac ttggtgaact gaaatatctc agtagccaaa
      241 ggaaaagaaa tcaaaagaga ttctgttagt agtgacgagc gaacacggaa caggctagtg
      301 gtttaaaggt aagaattaga atactctgga aagggtaacc atagaaggtg atagtcctgt
      361 ataagtggaa agtctttaaa tcctcgagta gggcgggaca cgtgaaatcc tgtttgaata
      421 tggggggacc atcctccaag cctaaatact ccttaacgac cgatagtgaa caagtaccgt
      481 gagggaaagg tgaaaagaac cctgggaagg gagtgaaata gaccctgaaa tcaagtgctt
      541 acaaacagtt ggagctctat gttgttccat gaaggaattt tgtggtatat acaacattag
      601 agtgaca
//