LOCUS       EY192996                 446 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa87h09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_491118.1| Putative cytoplasmic protein, with a
            coiled coil-4 domain, of eukaryotic origin [Caenorhabditis elegans]
            gb|AAF60772.2| Hypothetical protein Y54E10A.11 [Caenorhabditis
            elegans], mRNA sequence.
ACCESSION   EY192996
VERSION     EY192996.1  GI:159497125
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 154
            original value before phred recall for SR was 646
            PolyA tail found inside read...SR being truncated to 603
            Potential full insert read...vec-to-vec length: 518
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg ccccgaaggc ggagacgggg cgctccatcg tgagcaagga cctgcaccgc
       61 aggtggctgc tccagctgga agtgttcctc aacagacaga acggcgacat gttggacggt
      121 ctgttccagt ggaagcgcag catcgaccag cacttgcgcg gcgtggagga ctgcaccatc
      181 tgcatgtcca cggtgtccac cacaaactac caattgccga aaatccgatg tcgacagtgc
      241 aagaaaaagt ttcacggcga ttgtttgtac aaatggttcc aaaccagctc caagtcctcg
      301 tgtccacttt gccgggccaa ctttgtttga gacaactctc tggaagtgtc gacccaaaaa
      361 tgtgattgta ttttgctcac cccacccctc cattttctat cattttttcg gtgtttcctt
      421 agtttcgaat aaaatatttt gtttta
//