LOCUS       EY192994                 414 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa87h05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to emb|CAD44625.1| cathepsin B1 isotype 2 [Schistosoma
            mansoni], mRNA sequence.
ACCESSION   EY192994
VERSION     EY192994.1  GI:159497123
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 820 was extended to value 821 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 154
            original value before phred recall for SR was 612
            PolyA tail found inside read...SR being truncated to 569
            Potential full insert read...vec-to-vec length: 486
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga agccaacatg atcgtctact acgacttcat gttctacaag agcggcgtgt
       61 accagactgt gttcccgtgg ccgctgggcg gacacgcagt gcgcattgtc ggctggggcg
      121 tggacggccc gaccaaggtg ccatactggc tggtggccaa ctcgtggaac accgactggg
      181 gagaggacgg ctacttccgc atccaccgtg gcaccgacga gtcctacatc gagagctggg
      241 gcgtcaacta cgcgtcagcg atcgttgagt aaattggact gcaaggggca agaagattac
      301 aacacaaagc gaacatataa tttataacaa acacctttga tcatccatgt ttgtttgttc
      361 cttttaccag tttttttact tcgaagtccg attgaaataa agaagatgca ttca
//