LOCUS       EY192988                 550 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa87g11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to pir|T16646 hypothetical protein R01H2.6 - Caenorhabditis
            elegans, mRNA sequence.
ACCESSION   EY192988
VERSION     EY192988.1  GI:159497086
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 609 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 756
            PolyA tail found inside read...SR being truncated to 701
            Potential full insert read...vec-to-vec length: 622
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcac tggactgtga tgctctatcc ggaccgcgag ccatataaca aaggagcatt
       61 ccgtgtcacc gtctattttc cgacagaata cccatttaaa ccacctagag tcacattcca
      121 gacaaaaatc taccatccga atgttgacga caagggccaa gtttgtttgg ggatcatcca
      181 ggctgaaaat tggaagcctg caactcgaac tgagcaagtt atgaatgcct tgatgtctct
      241 gatctccgaa cctgagatcg atcatccgct gcgtggtgac ttggccgagg aatatcagaa
      301 ggacaaaaaa aagttcctca agaatgctga agatttcact cgaaagcacg cagaacgccg
      361 tgaataaacg accgagaaca gccagatgaa cgatgaacca acacattctg gaagttttgt
      421 ggtaaatcga atacggttga tcttttcttc caccgatcat ctcaaaattc tatataagtt
      481 attaaccatt tgacctttat tgttcgattg gtgacttcga tagcttatat ataaaatgct
      541 tcaaaaacca
//