LOCUS EY192987 346 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa87g10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192987 VERSION EY192987.1 GI:159497085 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 346) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 541 PolyA tail found inside read...SR being truncated to 498 Potential full insert read...vec-to-vec length: 418 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..346 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcct acttggataa ctgtggtaat tctagagcta atacatgcac aaaaactccc 61 aacgcaagga gggagcgcat ttgttcgcca caaaaccaac ccccctcggg ggttgagtgt 121 tgaatcagag caactaagct gagcgcacgg tcttgcaccg gcgccgtatc attcgagctt 181 ctgtcctatc aactttcgac ggtagtgtct ctggctaccg tggtggtgac gggtaacgga 241 ggatcagggt tcgactccgg agaaggggcc tgagaaatgg ccactatgtc taaggatagc 301 agcaggcgcg caaattaccc actctcagca tgaggaggta gtgaca //