LOCUS       EY192981                 531 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa87g03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192981
VERSION     EY192981.1  GI:159497049
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 815 was extended to value 816 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 717
            PolyA tail found inside read...SR being truncated to 681
            Potential full insert read...vec-to-vec length: 603
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaac tggcagtccg tcaaaagatc attgcgcttt tgaatccaca gcaaccagtc
       61 gatttgcgtc acctcgaagt catggcgctc ttgttcggat acatcaatgg tggcttccgt
      121 gcaggagtgg aagctgctgg gaatcctgga cttccgcttc tactcaacaa tatgttgaag
      181 gacgcgaaac tggcagcggc tcagacaatt gacgtggttc cctttatgca taccaacttt
      241 gtgacaatgc gaaactcgct gtccaacaaa tacaaccatt ttgtggacca aaacgaaatg
      301 gtgcaaaagc tcgaagcggt tgtgaacaat tctccggaat gggtgctgct tgggctttac
      361 acggagactg agcgaatgat tgctctgatt gccgatcgca tcgagggact gccgatggac
      421 ttggccaata actgaattga atgagcgaaa aatcggatga tctgatcctt tgttatcgtt
      481 atgttttaca gaagtacaga acagataaag gttaaaaaga attctcggcc a
//