LOCUS EY192971 232 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa87f04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192971 VERSION EY192971.1 GI:159497011 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 232) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 423 PolyA tail found inside read...SR being truncated to 383 Potential full insert read...vec-to-vec length: 304 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..232 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggg cttctgcgca gtggtcaaca agattgccgt gcgccgtgac gatgttgggc 61 cgattgagtt cttctaccgc tgaactgcat ggacagagat ctctgacttt tcacacaatt 121 tgacctcaat ctgtatgatc cggctctgtc cgatcattga tcgttattat tttaaaaaga 181 cacttctggt ttctggttat tttctcacca aagaattaaa cgaatttatc ta //