LOCUS       EY192969                 324 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa87f02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192969
VERSION     EY192969.1  GI:159497009
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 823 was extended to value 824 (,)
            putative full insert read: vector-to-vector length is 370 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 518
            PolyA tail found inside read...SR being truncated to 475
            Potential full insert read...vec-to-vec length: 396
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat tttttgtaat tatgatttta ggttataaat aattaatgaa gtataaacta
       61 ttaatgttat attttttaga taaattaatt tttctagtaa tttattttta gataaattaa
      121 tgttccagaa atatcggcta gacattatta tttttaacta aaaacttttt aaattttatt
      181 ttaatttata taaatttata ttaatatagg tgaaatttta attataatta tgttaataat
      241 tttataaaat ttaaaatttt aatttttaac ttaggttaga cactaattaa tgaaatttaa
      301 taattttctt tagtaaattt ttga
//