LOCUS       EY192966                 542 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa87e11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192966
VERSION     EY192966.1  GI:159497006
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 585 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 734
            PolyA tail found inside read...SR being truncated to 693
            Potential full insert read...vec-to-vec length: 614
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga ttcggtcgat ggaaagcgtt ttgcgtgcga tgtaaaagga gggataagtg
       61 atcgcttaaa gcagtggatg ctggctggta cacgtgtaaa tgtcaaactg agaggacacg
      121 ctcccaaccg ttctgtccaa gaaccgaatc gaatcattaa gggcgcaatc gtggcttatg
      181 ataggcactg gaacatggtg ttgcgcgatg tagacgaagt ctatttgccg gcttcgagaa
      241 tgagcaaggt ccaatccatg cctggtgcca ttccatctcc cgcgcgttac acattagata
      301 ggagtaagaa aggacgggct ttactcacac gcaacttaaa gtgctcaatg gtgactggtt
      361 catccgtgat tgcaatttac tgtgatgaac agcaatgaga agagtcgatt ccgtatattc
      421 ttatatcttt tacgtttaga tttgatgtat ttatagatgt cgcatcgcag gaaaaatgga
      481 ttctttccct ttgcgacata gatagatatg attgcataca gataaatata aaaacatcat
      541 ca
//