LOCUS       EY192958                 447 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa87e01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192958
VERSION     EY192958.1  GI:159496969
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 827 was extended to value 827 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 642
            PolyA tail found inside read...SR being truncated to 599
            Potential full insert read...vec-to-vec length: 519
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa cgctataaga cgcagctgtg caagaacatg tttcgacagg gggtgtgcaa
       61 attcggtgcc gactgttggt ttgcgcacaa tagtgccgaa cttcggaccc cgaattgtcc
      121 tctcgctgcc caaacgactg gccaacgctt cagtggatca actccgcatc cgtactctac
      181 caatcgcgtc tgggacaatc tgttctagca attcttacaa gggatcttaa attggaaatc
      241 aacaaagtcc aaacaagcat atttatcaat ttttctccct ctggaaactt catatatttt
      301 gaaaaatcac atccttatcc aatcatacaa gccagtttgt tttaacaatt tgccactttg
      361 agatccagaa agttgaaact atcacatatt tcttaataat actttattcc tggaaccctg
      421 gatttcgaat aaaaaataaa cggttga
//