LOCUS EY192947 231 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa87d02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192947 VERSION EY192947.1 GI:159496930 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 231) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 818 was extended to value 819 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 425 PolyA tail found inside read...SR being truncated to 382 Potential full insert read...vec-to-vec length: 303 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..231 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggagc gcaacagcga gttccgcatc accggttgtg tgctgctctc gctcggcttc 61 gcgggtctgg ccggctgcgc catcgtgcag cgcaagaaca tcggcaaact ctgggacgac 121 atgaccagag acctgttcgc ggtcggggag tgaggcagct ggcccaggtc gcccagaaag 181 gtgtgcctct ttgcctttcc attgtttaag ataaatcatg tttcccctcc a //