LOCUS       EY192945                 528 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa87c12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192945
VERSION     EY192945.1  GI:159496928
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 570 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 717
            PolyA tail found inside read...SR being truncated to 679
            Potential full insert read...vec-to-vec length: 600
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggccc agtgtgcgtc tgcagtcgca gactgaccaa aagttcgtga gcgcccagcg
       61 caacggggca gcaggcaaac agctgatgct gaaggcggtc gagccggacg agaagcgcgc
      121 cgacgcattt gcgctgatca acaacgcgga cgacggcact gtttcgctgc gccactcgga
      181 cggcgccttc gtgagcgtgt tcccgcgtgg cggacacctg atggttggac gcggcaaaga
      241 gcgcacgccc acccaaccgg aacgcttcaa catggcgaaa cagggcaacg gctacatcct
      301 gaagtccgtc tacaacgaca agtacgtggg cgtggacccg aagatggaca accgactcta
      361 cgccaaccag aaggaggcga gcggcgcaac tgtgttcgcg atggaccatt gagaagagaa
      421 gttaagaaga aaactgctaa tggagcgaaa aatgaagtga tcgcccgatt gacaatgatc
      481 gcacaatctt cacatatatt caatgcgaat ggataaaaat atggcgga
//