LOCUS       EY192940                 410 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa87c05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to gb|AAO43307.1| putative polyubiquitin [Arabidopsis
            thaliana], mRNA sequence.
ACCESSION   EY192940
VERSION     EY192940.1  GI:159496895
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 604
            PolyA tail found inside read...SR being truncated to 560
            Potential full insert read...vec-to-vec length: 482
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac cattaccctg gaagtcgagg gttctgacac catcgagaac gtcaaggcca
       61 agatccagga caaggaaggc attccgccgg accagcagcg tctgatcttc gccggaaagc
      121 agctcgagga cggtcgcacc cttgcggact acaacatcca gaaggaatcc accctccatc
      181 tggtgttgcg tcttcgcggt ggttccgcct gagtcagaga acgcgcagag gaatatggcg
      241 tcttgagatg tggaccaatt ttaattcctg ttctgatcac acaaaacaca atccacatgt
      301 tttacgatat acataacagc agaaaacaac ctacattcat taagtaatca tgctttctcg
      361 atatgtaatt ctgaaatggc tttcgcggaa cagtaaagaa tacaaaatga
//