LOCUS       EY192934                 495 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa87b11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to sp|Q25381|ACTM_LYTPI ACTIN, MUSCLE (LPM) gb|AAA53367.1|
            cytoskeletal actin, mRNA sequence.
ACCESSION   EY192934
VERSION     EY192934.1  GI:159496889
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 688
            PolyA tail found inside read...SR being truncated to 647
            Potential full insert read...vec-to-vec length: 567
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggct cctcctgagc gcaagtactc cgtctggatc ggaggctcca tcctggcctc
       61 gctgtccacc ttccagcaga tgtggatcag caagcaggag tacgacgagt cgggcccgtc
      121 catcgtgcac cgcaagtgct tctaagcagg gacagcggac agcgagacag acacagagga
      181 aggcagacag acagacaaca gaaaagtgct tcttttccac ccttccattt tttgcctgga
      241 agcttctttc ggagagaccc accatccacc acctttgttg ttcccatcca cacccaaccg
      301 ccacaaccca agatccatcc ccctcaccct cctgttgccg ctccctccgt ccctggccta
      361 gccgcgtaga ggggggagtc ggcccaattc ggcggcggat gatgtccaca ctgccatcca
      421 ttgtgcgcgt tgtttgtgtt ttggggacac tctttgccgt cgtcacgttt caaaggataa
      481 acccgattat ctgta
//