LOCUS EY192933 643 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa87b10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_500420.1| IQ calmodulin-binding motif and Sec7 domain containing protein [Caenorhabditis elegans] pir|T29902 hypothetical protein M02B7.5 - Caenorhabditis elegans gb|AAB09130.1| Hypothetical protein M02B7.5 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY192933 VERSION EY192933.1 GI:159496888 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 643) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 804 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..643 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa cggatcaaag aaaacgaatt ccaagcaggc acggaccatg tgacgcaagt 61 gatgaaagtg gaccagagca tagtcagcaa ggagaaaccg aagcttttgg agccgcaccg 121 tcgtctcatc tgctactgcc gtttacacca gatcaccgat gccaacaagc gccagtcttc 181 cggtgcgcat cagcgcgaaa tatttctctt caacgacctg gtgctggtgt gcaagctggc 241 tggacggaag cggctgaaca gcagtgccac ttccagcggc gcgggccagt atcaattgcg 301 ctgctggagt gctctgatcg gtcttcacat agaggagttc caaaacgagt tatacccaca 361 cggactggtg atcagctgcc cagacggaca agttctattg tttaatgcga agaacggaga 421 tgacagatat cgcttcgtgg cggacgtccg ggaatcggtg gccgagtcga ccgaaatgga 481 gcagtggcgt ctgaatttgg agttggacaa gcaatctctg gcgggcattg gcgctcggct 541 agccgattac aacttccata ttgcctcacg acatcaccat ccccccaatg atggcagcgt 601 tattgtgcac tcgagggcag gcaccagcgg attgatgaat cga //