LOCUS EY192924 331 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa87a12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192924 VERSION EY192924.1 GI:159496852 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 331) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 524 PolyA tail found inside read...SR being truncated to 483 Potential full insert read...vec-to-vec length: 403 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..331 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggagg agaacggaaa ttgcggacac aacgcaacag ccggaagagc aaatgtccca 61 gcggaacact gcctcaactc agctgaacac tccagcaaca gaaaaagcag aggtggagac 121 tgttcccgag accccgatgg atgacttcga ggaggaggac agcgtcgagt tttgagtgtt 181 cggtgaatga tgtttaattt tccatttcga gcattgctcc ctttgatcaa atgtttggcg 241 aaatactgat gttttatgca ttcccctcat ctctcgtttt ccattttggt tccaaacctt 301 tctgtgtgta atgaataaag aataccaatg a //